Background :
@EN Various molecular biological techniques using fetal cells in maternal blood have been recently developed as a noninvasive method for prenatal diagnosis. Current technologies, however, have not gained a sufficient diagnostic accuracy for
routine
use
because only a few nuber of fetal cells exist in maternal circulation. The process of cell enrichment is required to overcome this problem.
@ES Objective :
@EN The purpose of this study was to compare the usefulness of cell sorting methods according to single or multiple use of monoclonal antibodies in the detection of Y-specific sequence(DYS14) from fetal nucleated red blood cell(nRBC) in maternal
blood.
@ES Study Design :
@EN periperal blood was obtained from eighty-one pregnant women at 11-24 weeks of gestational age. Fetal nRBCs were discriminated by means of centrifugation under triple density gradient and by magnetic-activated cell sorter(MACS) using CD45
monoclonal
antibody(mAb). Thirty-nine samples were resorted using CD 71 and GPA mAb after using CD 45 mAb. After DNA extraction, polymerase chain rection(PCR) and gel electrophoresis were performed for the detection of Y-specific sequence(DYS14) in each
sample.
@ES Results :
@EN The diagnostic indices of PCR after cell sorting only CD45 mAb in the detection of Y-specific sequence from fetal nRBC in maternal blood were sensivity 85%(17/20), specificity 86%(19/22), positive predictive value 85%(17/20), and negative
predictive
value 86%(19/22). After using multiple monclonal antibodies, those of PCR were 68%(13/19), 85%(17/20), 81%(13/16), AND 74%(17/23), respectively.
@ES Conclusion :
@EN Cell sorting method using multiple monoclonal antibodies may not add more efficacy in the detection of Y-specific sequence of fetal nRBC.
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